Establishment and characterization of FusionRed stable transfected canine prostate adenocarcinoma and transitional cell carcinoma cells

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In Vivo. 2022 Jan-Feb; 36 (1): 170-179. doi: 10.21873 / invivo.12688.

ABSTRACT

BACKGROUND / OBJECTIVE: Cancer cell inoculation is commonly used to evaluate new therapeutic approaches in vivo. However, without reporter genes allowing deep tissue imaging, the study of early tumor progression and therapeutic responses is often limited. We describe the establishment and characterization of two canine cancer cell lines stably expressing red fluorescent proteins as tools for subsequent in vivo imaging.

MATERIALS AND METHODS: Two red fluorescent cell lines were generated by plasmid transfection. Expression of the fluorescence protein was confirmed by flow cytometry and microscopy. Deep tissue imaging was demonstrated in mice using a NightOWL LB 983. Changes in gene expression after transfection were analyzed by RNAseq.

RESULTS: Both cell lines were detectable in vivo by subcutaneous injection of 1 × 106 cells. RNAseq revealed until 2005 differentially expressed genes induced by transfection, but no significant changes in key cellular pathways.

CONCLUSION: Fluorescent cell lines provide a solid basis for future in vivo studies in canine cancer.

PMID: 34972712 | DOI: 10.21873 / invivo.12688


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